For me, it would be GLaDOS.
Portal 2 spoiler
She deleted the one remaining human part of herself because it was just too much to deal with. She releases you at the end of Portal 2 as a path of least resistance measure because “killing you is hard.”
What about you, what is your favorite media villain?
I feel like everyone is a villain in that series.
And I think that’s because everyone is very well written and has a full profile rather than being one-or-two dimensional good/evil.